Dyelabeled dna fragments can be analyzed on the applied biosystems 3730xl dna analyzers. From sample prep to data analysis, we provide expert solutions and dedicated support. This listing is for a this listing is for biosystems abi 3730xl 96capillary dna analyzer complete with dell. This kit contains four tubes of hsp69 standard sequence used for instrument and spectral calibrations. Doing so may cause the 37303730 xl data collection software to malfunction. How long can i keep the polymer on the applied biosystems 37303730xl dna analyzer. Dna sequencing is performed using applied biosystems automated 3730xl dna analyzers. Full service dna sequencing institute of biotechnology. There are a variety of sequencing and fragment analysis software available to view results. The cfx 96 instrument has optional software to run and interpret high resolution melt curves for allelic descrimination.
Used applied biosystems 3730 analyzer dna related for sale. Applied biosystems 3730xl user manual pdf download. Applied biosystems 3500 series genetic analyzers for dna. Researchers use applied biosystems integrated systems for sequencing, flow cytometry, and realtime, digital and end point pcrfrom sample prep to data analysis. Audience this guide is intended for novice and experienced users who analyze. Applied biosystems integrated systems for sequencing and realtime, digital and endpoint pcr have been trusted by the worlds top scientists for over 20 years. View and download applied biosystems 3730xl user manual online. With the 3730xl dna analyzer, you get the highestquality data from a wide array of applications. The dye terminator mixture contains normal dntps and dye terminator.
Applied biosystemsabi 3730 dna analyzer equipment used. With the 3730 dna analyzer, you get the highestquality data from a wide array of applications. The abi 3730xl dna sequencer is the flagship model of applied biosystems sanger sequencer line, engineered to deliver the highest throughput and the fastest turnaround time for all sequencing and. Plasmids were diluted to 100 ngul and 10 ul of the resulting solution mixed with the sequencing primer.
Versatile and innovative, our genetic analyzers have been designed with you in mind to meet the demands of your genetic testing needs. Thermo fisher scientific provides wide range of products under applied biosystem brand which includes 37303730xl dna analyzer sequencing standards, bigdye terminator v3. The uccccdsf uses two applied biosystems 3730xl 96capillary and one. Page 1 applied biosystems 35003500xl genetic analyzer user guide with 3500 series data collection software 3. Applied biosystems 3730xl dna analyzer thermo fisher scientific. The applied biosystems 3730 and 3730xl dna analyzers include. Analyzers and 33xl genetic analyzers ab navigator software administrator guide.
Refurbished applied biosystems abi 3730xl 96capillary dna analyzer mn 6250020 complete with dell pc workstation, new lcd monitor, keyboard, mouse, 3730 family data collection software, 3730xl sequencing analysis application software, 96well septa plate kit, 96well tray, 3730 sequencing chemistry guide, all necessary cables and manuals. Sequencing was performed using a proprietary chemistry based on big dye 3. Feb 17, 2010 applied biosystems 3500 series genetic analyzers for dna sequencing and fragment sizing. Applied biosystems genetic analysis data file format. Software university core dna services university of calgary.
Compared to the previous generation abi 3700 sequencer, the 3730xl offers. Instructions and examples in this guide were generated with applied biosystems sequence scanner software, available for free download following online registration. Comprehensive cancer center dna sequencing facility. Strongly recommended for all users with windows pcs. Pcr amplification of a dyelabeled primer is the most widely used method for incorporating dyes in dna. All instruments use a 96well plate format for quantitative pcr. Dna shearing and size selection covaris s220 ultrasonicator holders and tubes for 50 ul to 2ml samples useroperated with training. Find the best price on a widevariety of new and used abi 3730xl.
Feb 10, 2016 abi 3xl sequencer available for sale at the lab world group. In addition to being ideal for highthroughput sequencing, the analyzers optimized application assays, instrument, and analysis software also provide a complete. The applied biosystems 3730 and 3730xl dna analyzer includes patented technology licensed from. Pcr amplification of a dyelabeled primer is the most widely used method for incorporating dyes in dna fragments. Software university core dna services university of.
The cfx 96 is capable of multiplexing up to five different fluorphores providing the capability of multiple taqman probes. Davis sequencing offers a full dna sequencing service. Samples for sequencing are generated in a reaction like pcr but with only a single primer present. With the dna sanger sequencing ready to run, the user submits the. Applied biosystems abi 3730xl 96capillary dna analyzer. University of chicago comprehensive cancer center dna sequencing and genotyping facility. The applied biosystems 3730xl dna analyzer can be used for dna fragment analysis applications such as microsatellites, aflp, snp analysis, mutation detection and traditional dna. The ab 3730xl is a fully automated system used for detecting. Dna sequencing applied biosystems bigdye terminator v3. The software analyzes, displays, edits, saves, and prints sample files that are generated from applied biosystems dna analyzers and genetic analyzers.
Applied biosystems abi 3730xl 96capillary dna analyzer iet. Applied biosystems genetic analysis systems continue to offer proven highquality instrumentation, reagents, consumables, analysis software. We use applied biosystems 3730 and 3730xl dna analyzers to generate the best possible data for our customers. Dna sequencing by capillary electrophoresis by applied biosystems. First, the accompanying software package is intuitive and comprehensive. Information about the 3730xl instrument and how samples are prepared to run on it can be found in the applied biosystems 3730 3730xl sequencing chemistry guide. In addition to being ideal for highthroughput sequencing, the analyzers optimized application assays.
Solid 5500xl sequencing by oligonucleotide ligation and detection developed by life technologies and made available in 2006, the solid 5500xl sequencer is the only next generation platform to sequence via primer ligation. The ab1 file is a file type produced by data collection software generating sequencing data, with the extension. The dye terminator mixture contains normal dntps and dye terminator dntps, which are missing an oh group at the 5 end and instead have a dyelinkerdye molecule that is different for each of the four bases. The applied biosystems veriti thermal cycler delivers the proven reliability you expect from applied biosystems pcr instruments. Network licenses for the most common sequence analysis software like mac. System is complete with computer and original disks. We use big dye terminator chemistry and amplitaqfs dna polymerase. Guided tour of applied biosystems quantstudio 12k flex system duration. The veriflex temperature control technology inside makes it possible to. Applied biosystems is one of the various brands under the life technologies brand of thermo fisher scientific corporation. Applied biosystems 3730xl 96capillary computer w preloaded existing software data.
Starting the 37303730xl system applied biosystems 37303730xl dna analyzers user guide 17 starting the 37303730xl system about naming the computer do not rename the computer once 37303730xl dna analyzer data collection software has been installed. Guided tour of applied biosystems quantstudio 12k flex system. Sanger sequencing on abi 3730 perelman school of medicine. Sequencing with the applied biosystems 3730xl university core. Applied biosystems 3730 3730xl dna analyzer data collection software has been installed. Ab sanger sequencing guide center for genome research. Applied biosystems 37303730xl dna analyzer getting started guide. Doing so may cause the 37303730xl data collection software to malfunction. Applied biosystems genetic analyzers 310, 3100, 3xl, 3500xl, 3730xl. You may need to repeat data extraction andor data analysis. Applied biosystems 3500 series genetic analyzers for dna sequencing and fragment sizing. Data collection supplied with the instrument manages your instrument setup, controls instrument operations, allows realtime data visualization, and performs diagnostics. Both standard and high throughput sanger dna sequencing samples are run on the facilitys applied biosystems 3730xl dna analyzer.
This latest version of 3730xl dna analyzer has an upgraded solidstate laser, is 48 and 96capillary. Applied biosystems 3730 3730xl dna analyzers user guide vii preface about this guide audience this manual is written for principle investigators and laboratory staff who are planning to operate and maintain the applied biosystems 3730 3730xl dna analyzers. The applied biosystems 3730 and 3730 xl dna analyzer includes patented technology licensed from hitachi, ltd. Chapter 4 setting up the software for dna sequencing. Instructions and examples in this guide were generated with applied biosystems sequence scanner software, available for free download.
At the 7th annual advancing genome biology and technology agbt meeting, applied biosystems, an applera corporation business, introduced its targetseq resequencing system, a new software system for highthroughput directed or medical sequencing designed for use with applied biosystems 3730 and 3730xl dna analyzers. Refurbished applied biosystems abi 3730xl 96capillary dna analyzer mn 6250020 complete with dell pc workstation, new lcd monitor, keyboard, mouse, 3730 family data collection software, 3730xl. The applied biosystems 12k flex realtime pcr system is the best combination of convenience, flexibility, throughput, and costeffectiveness for middensity genotyping and gene expression studies. Applied biosystems dna sequencing analysis software. Data collection supplied with the instrumentmanages your instrument setup, controls instrument operations, allows realtime data visualization, and performs diagnostics. While in the past customer support has not been that good, it has gotten a lot better since 2005. Applied biosystems instruments, assays, and reagents have been trusted in the lab for over 20 years. Sequencing analysis software or seqscape software will not be performed. Applied biosystems is the leader in the dna sequencing market. Applied biosystems 37303730xl dna analyzers user reference manual 4331466 applied biosystems 37303730xl dna analyzers user guide 4331468 analyzing dna sequencing data abi prism dna sequencing analysis software v. May 01, 2006 the abi 3730xl dna analyzer offers an alternative in the form of a fully automated system. The abi 3730xl dna sequencer is the flagship model of applied biosystems sanger sequencer line, engineered to deliver the highest throughput and the fastest turnaround time for all sequencing and genotyping projects.
Applied biosystems 3730xl dna sequencer used lab equipment. The ab 3730xl is a fully automated system used for detecting fluorescently labeled dna fragments and can provide up to 800 bases of highquality dna sequencing data per reaction. A versatile trace viewing package from applied biosystems that allows users to inspect, edit, and print their sequencing data from the. Applied biosystems dna sequencing analysis software verion 5. Heatseal plates for the 37303730xl instruments if you are preparing multiple. Sequencing by oligonucleotide ligation and detection developed by life technologies and made available in 2006, the solid 5500xl sequencer is the only next generation platform to sequence via. Aug 28, 2005 in any event, kerfeld adds, most biology textbooks describe sequencing using slab gel systems, not capillaries. The abi 3730xl dna sequencer is the flagship model of applied biosystems. Base calling was performed using applied biosystems sequencing analysis software version 5. Sequencing analysis software uses a basecaller algorithm that performs base calling for pure and mixed base calls. Applied biosystems dna sequencing analysis software v5. In any event, kerfeld adds, most biology textbooks describe sequencing using slab gel systems, not capillaries.
Sequencing analysis lies within education tools, more precisely science tools. Applied biosystems quantstudio 12k flex realtime pcr. This listing is for a this listing is for biosystems abi 3730xl 96capillary dna analyzer. The applied biosystems analysis software is a secure web application for analysis of data that are generated on thermo fisher scientific realtime pcr instruments. The applied biosystems 3730 and 3730xl dna analyzers include patented technology licensed from hitachi, ltd. In addition to being ideal for highthroughput sequencing, the analyzers optimized application assays, instrument, and analysis software also provide a complete solution for genotyping and resequencing. The applied biosystems 3730 and 3730xl dna analyzer includes patented. Users provide dna plasmid, phage or pcr product at a standardized concentration and custom primers if necessary, and the facility performs cycle sequencing reactions using fluorescent dye terminators, runs the capillaries, acquires the data, and provides the sequence as chromatogram and text files. Several genetic analysis experiments incorporate the dye in dna fragments to gather critical information about the organisms. Be sure to start the computer workstation before starting the applied biosystems 3730 3730xl dna analyzers. Applied biosystems optimizes throughput of 3730 series dna.
The facility uses two abi applied biosystems sequencers. Table 2 lists applied biosystems software products for analyzing sequencing. Applied biosystems abi3730xl dna analyzer buy 3730xl,abi. Sequencing analysis software or seqscape software will not be. Applied biosystems genetic analysis data file format introduction this document is intended for programmers or bioinformatics groups who wish to perform additional anal ysis or other manipulation. Applied biosystems genetic analysis data file format introduction this document is intended for programmers or bioinformatics groups who wish to perform additional anal ysis or other manipulation of ab1 andor fsa files. At the 7th annual advancing genome biology and technology agbt meeting, applied biosystems, an applera corporation business, introduced its targetseq resequencing system, a new software system. Sanger sequencing and fragment analysis software thermo.
The university core dna genetic analysis laboratory uses automated sequencing methods with an applied biosystems 3730xl 96 capillary dna analyzer. Dna isolation we utilise commercial dna purification kits promega, wizard sv 96tm combined with robotic extraction qiagen, biorobot 9600tm. The protocol involves a pcr reaction using a very robust mastermix bigdye from applied biosystems, reaction purification and plate loading on the 3730xl. Sequencing with the applied biosystems 3730xl university. The actual developer of the software is applied biosystems. The 3730xl dna analyzer software suite allows you to generate more meaningful data with less work. Applied biosystems 37303730xl dna analyzer user guide pub. The automated dna sequencer the scientist magazine. After editing, sequence data is blasted in the ncbi genebank for identification, data mining or is aligned against reference sequences by using different software. Dna isolation we utilise commercial dna purification kits promega. Users provide dna plasmid, phage or pcr product at a standardized concentration and custom primers if necessary, and the facility performs cycle sequencing reactions using fluorescent. Ab sanger sequencing guide oregon state university. Sanger sequencing iowa state university dna facility isu. Applied biosystems genetic analyzers are a trusted standard for sanger sequencing and fragment analysis by capillary electrophoresis ce.
996 280 1385 244 959 213 456 1182 735 116 742 899 1549 106 833 1420 1475 1602 115 1185 1444 246 833 903 1620 922 1153 1257 62 89 1632 1427 956 1387 63 63 1250 1161 1073 1406 945 429 1053